I need assistance with Lab attached

I need assistance with Lab attached

I need assistance with Lab attached
Snork Synthesis Lab Lab Directions This activity, modified from the original at The Biology Corner, will help you practice your understanding of protein synthesis. Submit your lab answers according to the directions and grading rubric below. Overview: In this simulation, you will examine the DNA sequence of a fictitious organism, the Snork. Snorks were discovered on the planet Dee Enae in a distant solar system. Snorks only have one chromosome with ten genes on it. Your job is to analyze the genes of its DNA and determine what traits the organism has and then create a sketch of the organism (You can be creative here). Goal: Transcribe and translate all 10 genes for one snork. Then, draw a picture of your snork based on your work. Directions: For simplicity, the gene sequences are much smaller than real gene sequences found in living organisms. Each gene has two possible versions that result in a different trait being expressed in the snork. Example: the gene for legs can result in either 2 or 4 legs Snork Genes Amino acid sequence (abbreviations used) Trait expressed (outward appearance) 1 Body Hair val – ser – leu val – ser – lys hairless hairy 2 Body Style tyr – pro – glu – glu – lys val – pro – thr – glu – lys plump skinny 3 Legs leu – leu – leu – pro leu – leu – ser – ala 2 legs 4 legs 4 Head Shape ala – val – val val – ala – ala round head square head 5 Tail his – ile his – his tail no tail 6 Body Pigment ser – pro – val val – phe – tyr blue pigment (hair/skin) red pigment (hair/skin) 7 Eyes asp – ile – leu – leu – pro – thr asp – ile – pro – pro – pro – thr small slanted eyes large round eyes 8 Mouth val – asp – asp – ala asp – asp – asp – ala circular mouth rectangular mouth 9 Ears phe – ser – gly phe – phe – gly pointed standing-up ears rounded floppy ears 10 Arms arg – tyr – cys – lys arg – arg – asp – thr long spaghetti like arms short stumpy arms Choose a snork below (do not choose our example, Snoopy.) Transcribe the DNA into mRNA. Translate the mRNA given into an amino acid sequence to determine the trait (how the organism looks) based on the sequence. (Use the codon wheel or table given to you in the content to do this step.) Draw your Snork! First, let’s try an example. You can see Snoopy’s DNA below. Each gene is in order (1-10) and is separated by a slash to keep you organized. Snoopy Snork DNA: CAT AGG GAG / ATG GGG CTC CTT TTT / AAT AAT GAC GGG / CGA CAA CAT / GTA TAA / AGA GGG CAT / CTA TAA GAA GAC GGG TGT / CAA CTA CTA CGG / AAA AGA CCA / GCG GCA CTG Transcribe the DNA into mRNA. Remember that there are no T’s in mRNA, use U’s instead. Example: if the DNA codon reads CAT, the matching mRNA codon is GUA. Snoopy Snork mRNA: GUA UCC CUC / UAC CCC GAG GAA AAA / UUA UUA CUG CCC / GCU GUU GUA / CAU AUU / UCU CCC GUA / GAU AUU CUU CUG CCC ACA / GUU GAU GAU GCC / UUU UCU GGU / CGC CGU GAC Now, use your codon wheel or table to find the amino acid that matches each codon. *Note that abbreviations were used for amino acids.* (This table is enlarged below for your reference.) Write down the amino acids in order for each gene, as shown. Then compare each protein (amino acid chain) to the trait table above to find out the appearance of your Snork! Amino Acid Sequence: 1: val, ser, leu ( = hairless in table above) 2: tyr, pro, glu, glu, lys (= plump) 3: leu, leu, leu, pro (= 2 legs) 4: ala, val, val (= round head) 5: his, ile (= tail) 6: ser, pro, val (= blue) 7: asp, ile, leu, leu, pro, thr (= small, slanted eyes) 8: val, asp, asp, ala (= circular mouth) 9: phe, ser, gly (= pointed ears) 10: arg, arg, asp (= short arms) Now draw your Snork using the information you translated! (Sample drawing courtesy of a young biologist!) Now, you will choose one of the following Snorks and do the same thing! Snicker Snork DNA: CAG TCG GAA / CAA GGG TGT CTT TTT / GAG AAT TCA CGC / CAA CGC CGA / GTG TAA / AGA GGG CAT / CTA TAA GGG GGG GGG TGG / CTA CTG CTG CGT / AAG AAA CCC / GCG GCC CTG Snuffle Snork DNA: CAT AGA TTT / CAA GGA TGA CTT TTC / GAA GAG GAG GGG / CAA CGC CGA / GTA GTG / CAT AAA ATA / CAT TAA GAA GAC GGG TGT / CAA CTG CTG CGT / AAG AGC CCA / TCT ATA ACA Snapple Snork DNA: CAG TCG TTT / ATG GGG CTT CTC TTT / GAG AAT TCA CGC / CGA CAA CAC / GTA GTA / CAA AAA ATG / CTA TAG AAT GAC GGG TGG / CTG CTG CTA CGG / AAA AGA CCC / TCT ATA ACA Be sure to double check your work so that you do not make any silly mistakes! mRNA Table with Amino Acid abbreviations Data/Results 1. Which snork did you select for this activity? 2. What was it’s mRNA nucleotide sequence? Please include spaces and slashes as you see in DNA. 3. What was it’s amino acid sequence? Include slashes or write each of 10 characteristics on separate lines. 4. Include an original picture of your snork with the 10 labeled characteristics. You may hand-draw your snork and take a picture/scan it in, or you can create it using a drawing program (see the list of options in the sidebar in the content.) Grading: The following criteria will be used to evaluate your lab: Criteria Points Possible Identifies Snork Chosen 10 Results DNA sequence is correct and can be clearly read Amino acid sequence is correct and can be clearly read 40 Picture Original 10 labeled characteristics Accurate, matches amino acid sequence Hand-drawn and scanned or computer-generated 50 TOTAL 100 Snork Synthesis Lab 5 of 5

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